產(chǎn)品簡介
Catalogue Number ENZ-308 Synonyms DNA polymera
公司簡介
上海亞培生物科技有限公司，是一家專注于生命科學(xué)和生物技術(shù)領(lǐng)域的高科技企業(yè)，專業(yè)提供分子生物學(xué)、免疫學(xué)、生命科學(xué)基礎(chǔ)研究以及臨床檢測等諸多領(lǐng)域的試劑、耗材、儀器等各類產(chǎn)品及生物技術(shù)服務(wù)。 公司一方面注意跟蹤世界范圍分子生物學(xué)、細(xì)胞生物學(xué)等生物學(xué)科的發(fā)展前沿，努力將歐美專業(yè)生產(chǎn)廠家的具有國際先進(jìn)水平的產(chǎn)品推薦給各類研究人員；另一方面也非常重視自主產(chǎn)品研究和開發(fā)，推出了一系列具有競爭力的產(chǎn)品和服務(wù)。公司客戶遍布大學(xué)、研究所、醫(yī)院、衛(wèi)生防疫、商品檢驗(yàn)檢疫、制藥公司、生物技術(shù)公司和食品工業(yè)等單位。 公司在重視產(chǎn)品質(zhì)量的同時，也建立了一套集技術(shù)支持、物流、售后服務(wù)等多個部門的全方位的服務(wù)體系，努力把我們方便、快捷、周到的服務(wù)提供給每一個客戶。我們的目標(biāo)是讓亞培成為在生命科學(xué)和生物技術(shù)領(lǐng)域內(nèi)集生產(chǎn)、研究開發(fā)和銷售于一體的國內(nèi)外知名的一流企業(yè)每一個亞培人，都心存一個信念：希望通過自身的不懈努力，通過我們優(yōu)秀的產(chǎn)品、專業(yè)的服務(wù)來更好的服務(wù)用戶，為我們每一個用戶的成功鋪路搭橋，也為亞培的成功鋪路搭橋！
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產(chǎn)品說明
| Catalogue Number |
ENZ-308 |
| Synonyms |
DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1. |
| Description |
Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions. |
| Source |
Recombinant e.coli contains Thermus aquaticus polymerase gene. |
| Formulation |
Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20. |
| Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10?g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. 10X Reaction Buffer with MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.
10X Reaction Buffer without MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. |
| Include |
: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution. |
| Features |
Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction ConditionsChoose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested EnzymeOur PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account. |
| Applications |
PCR, 3´ A-tailing of blunt ends, compatible with Vectors. |
| Storage Conditions |
Stable for 5 days at 10°C, for longer period of time store at -20°C. |
| Storage Buffer |
Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme.
50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100. |
| Quality Control Tests |
PCR, activity, SDS-PAGE/purity, endonuclease/nickase. |
| Usage |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » Pfu DNA Polymerase |
| » Taq Plus DNA |
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