Catalogue Number CYT-201 Synonyms Erythropoiet

Catalogue Number	CYT-201
Synonyms	Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Introduction	This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Description	Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 30,400 Dalton. EPO-a is purified by proprietary chromatographic techniques.
Source	Chinese Hamster Ovary Cells(CHO).
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Each mg of lyophilized powder contains 0.58 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
Solubility	It is recommended to reconstitute the lyophilized Epoetin -a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity	The Specific Activity was measured by Normocyth-aemic mice and was found to be 120,000 IU/mg corresponding to a Specific Activity of 8.4ng/ml.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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